Annegret Potthoff and Wolfgang Unger (2019), Report on SOP for dispersing nanomaterials in water and report on round robin test.



This report describes a standard operation procedure for dispersion of nanomaterials in liquids prior to toxicological or ecotoxicological testing. Main factors, who determine the state of agglomeration and aggregation of ENMs after dispersion are specific energy input, particle concentration and fluid composition. The method was validated in two round robin tests, where two typical nanomaterials (a nanopowder and a nanodispersion) were investigated. One main result arising out of the data comparison was that only those participants, who were able to follow the instructions in the SOPs completely, received similar results regarding particle size and zeta potential. The SOP is easy to adapt for other types of nanomaterials. The results provide the standardization process and were presented at DIN working group.


nanoparticle; particle size; zeta potential; round-robin test

Project Data:

  • NanoValid: Development of reference methods for hazardidentification, risk assessment and LCA of engineered nanomaterials

  • Theme 4: Nanosciences, Nanotechnologies, Materials and new Production Technologies and Theme 6: Environment (including Climate Change)

  • Contributors (co-authors with participant short name): NICPB; INMETRO; EAWAG; Nanologica; UoB; IMT; Veneto; BAM

  • Comment: The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under Grant Agreement n° 263147